New approaches for single cell genome sequencing and mutation analysis

Recorded On: 02/06/2018

Microfluidics and whole-genome amplification are enabling single-cell genomic analyses.  At the same time, these technologies limit single-cell genomic studies by imposing cost and complexity (microfluidics) and degrading data quality (whole-genome amplification). Here I will present two new methods for single-cell genome analysis, one that requires no microfluidics or specialized equipment for direct single-cell genome amplification and another that leverages culture-based amplification rather than biochemical amplification to enable studies of de novo mutations in single cells.

Paul Blainey

MIT Department of Biological Engineering and Broad Institute of MIT and Harvard

Dr. Blainey trained in mathematics, chemistry, biophysics, microfluidics, and genomics before joining the Broad Institute and the Department of Biological Engineering at MIT as a faculty member in 2012. Dr. Blainey’s laboratory integrates microfluidic, molecular, and imaging tools to address new challenges in single-cell analysis, genomic screening, and therapeutics development.

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