Traditional methods for 3D cell culture are often time consuming, display variability, and lack the necessary throughput for screening applications. To address these concerns we have developed a set of assay plates and flask formats that enable highly reproducible formation and screening of spheroids. In this talk we highlight through a series of case studies how these tools enable a variety of applications including organoid models, immune oncology, and hepatotoxicity models. Specifically, we show how a novel combination of Spheroid and Transwell plates enables the investigation of immune cell homing, tumor cytotoxicity, and tumor immune evasion in an easy-to-use 3D high throughput assay. We highlight the formation of gastrointestinal organoids derived from human induced pluripotent stem cells. And we demonstrate how primary human hepatocytes models can be improved with these tools to more closely correlate with in vivo toxicity data.
Dr. Anthony G. Frutos was appointed business technology director, Corning Life Science Development in April 2009. In this role, Tony is responsible for Life Sciences product and process development and delivery. Tony holds 19 U.S. patents and is an author on more than 39 technical publications. He holds a bachelor’s degree in chemistry from Brigham Young University and a Ph.D. in analytical chemistry from the University of Wisconsin, Madison.