Development of an Automated High Throughput CHO Stable Pool Platform for Generating Large Protein Collections

Recorded On: 02/05/2018

Recombinant protein expression and purification is a central process in biomedical research and Chinese hamster ovary (CHO) cells are a primary workhorse for protein production from mammalian cells.  GNF has developed a robust suite of software and automated systems to support high throughput CHO (HT-CHO) stable pool establishment, archive of cell banks and protein purification.  Pools are established in 96-well plates, maintained until they are ready for scale up, and then expanded into an AutoFlask™.  Once cells reach the desired density, cell bank archives are created and one or more batch production AutoFlasks™ are inoculated depending on the amount of protein requested.  As an example, a single 50mL culture expressing a human IgG1 antibody typically yields 10 milligrams of protein.  Innovative solutions ranging from a new software Dashboard to manage projects and execute processes, a recently developed non-invasive Flask Density Reader and an upgraded harvest and purification system compatible with magnetic beads will be presented.  This platform enables cost-effective, facile production of proteins at quantities and quality useful for early stage drug discovery tasks such as screening, protein engineering and even in vivo studies. 

Paul Anderson

Gnf Systems

BS Chemical Engineering, UCSB, 2003; MS Biomedical Engineering, Case Western Reserve University, 2005; Senior Automation Engineer, Genomics Institute of the Novartis Research Foundation, 2005-Present

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